Expression of human CYP27B1 inEscherichia coliand characterization in phospholipid vesicles
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چکیده
منابع مشابه
Binding of human factor VIII to phospholipid vesicles.
Factor VIII, a protein cofactor involved in blood coagulation, functions in vitro on a phospholipid membrane surface to greatly increase the rate of factor X activation by factor IXa. Using gel filtration, rapid sedimentation, and resonance energy transfer we have studied the interaction of recombinant-derived human factor VIII with small and large unilamellar phospholipid vesicles composed of ...
متن کاملCholesteryl ester transfer from phospholipid vesicles to human density lipoproteins.
The exchange of cholesteryl esters between different lipoproteins was reported to bae mediated by a protein present in human plasma. In this study wer have examined the movement of cholesteryl ester from unilamellar phospholipid vesicles to high density lipoprotein (HDL). Experimental conditions were establisehd so that vesicles containing egg yolk lecithin and cholesteryl oleatea (molar ratio ...
متن کاملReconstitution of rabbit thrombomodulin into phospholipid vesicles.
The influence of phospholipid on thrombin-thrombomodulin-catalyzed activation of protein C has been studied by incorporating thrombomodulin into vesicles by dialysis from octyl glucoside-phospholipid mixtures. Thrombomodulin was incorporated into vesicles ranging from neutral (100% phosphatidylcholine) to highly charged (30% phosphatidylserine and 70% phosphatidylcholine). Thrombomodulin is ran...
متن کاملInteraction of microtubule proteins with phospholipid vesicles
We have examined the interaction of unilamellar dimyristoyl phosphatidylcholine liposomes with the high-speed supernate of brain homogenate and with tubulin purified through one or two cycles of microtubule assembly-disassembly. Tubulin and certian high molecular weight proteins are selectively adsorbed from these mixtures onto liposomes. The composition of adsorbed proteins is similar to that ...
متن کاملThe phospholipid flippase activity of gastric vesicles.
We found that isolated gastric vesicles contain a novel Mg2+-ATP-dependent phospholipid translocation (flippase) activity. Fluorescence analogue of phosphatidylcholine, 2-(12-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl)amino)dodecanoyl-1-hexadecanoyl-sn-glycero-3- phosphocholine, was ATP-dependently translocated from the outer (cytosolic) to inner (luminal) leaflet of the lipid membrane bilayer of hog ...
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ژورنال
عنوان ژورنال: The FEBS Journal
سال: 2012
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2012.08736.x